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pjbei 6409 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pjbei 6409 plasmid
    Pjbei 6409 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pjbei/pJBEI-6409+(Plasmid+%2347048)/pm41619942-71-11-13
    Average 93 stars, based on 21 article reviews
    pjbei 6409 plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Enhancing Production of Pinene in Escherichia coli by Using a Combination of Tolerance, Evolution, and Modular Co-culture Engineering
    Article Snippet: .. pMEVI was derived from pJBEI-6409 (Alonso-Gutierrez et al., ), which was obtained from Addgene. pJBEI-6409 contains six genes of the MEV pathway ( atoB from E. coli, HMGS , and HMGR from Staphylococcus aureus and MK, PMK , and PMD from Saccharomyces cerevisiae, idi from E. coli, GPPS from A. grandis , and limonene synthase gene ( LS ) from Mentha spicata ). ..

    Article Title: Enhancing Production of Pinene in Escherichia coli by Using a Combination of Tolerance, Evolution, and Modular Co-culture Engineering.
    Article Snippet: .. pMEVI was derived from pJBEI-6409 (Alonso-Gutierrez et al., 2013), which was obtained from Addgene. pJBEI-6409 contains six genes of the MEV pathway (atoB from E. coli, HMGS, and HMGR from Staphylococcus aureus and MK, PMK, and PMD from Saccharomyces cerevisiae, idi from E. coli, GPPS from A. grandis, and limonene synthase gene (LS) from Mentha spicata). ..

    Article Title: Bacterial Production of Pinene by a Laboratory-Evolved Pinene-Synthase.
    Article Snippet: Successful feeding of the substrate geranylpyrophosphate (GPP) to monoterpene synthase is critical to the efficient microbial production of monoterpenes.. Over-expression of GPP synthases, metabolic channeling from GPP synthase to terpene synthases, and down-tuning of endogenous competitors have been successfully used to increase the production of monoterpene synthesis.. Nevertheless, the production of monoterpenes has remained considerably lower than that of hemi-/ sesqui-terpenoids.

    Construct:

    Article Title: Heterologous expression of 2-methylisoborneol / 2 methylenebornane biosynthesis genes in Escherichia coli yields novel C11-terpenes
    Article Snippet: E . coli strain DH5α (New England Biolabs) was used for plasmid construction. .. E . coli MG1655 Δ endA Δ recA (DE3) (hereinafter referred to as E . coli MG1655) [ ] was constructed in the lab of Kristala Prather (Addgene #37854) and used for production experiments. pJBEI-6409 was constructed in the lab of Taek Soon Lee (Addgene plasmid # 47048). ..

    Plasmid Preparation:

    Article Title: Heterologous expression of 2-methylisoborneol / 2 methylenebornane biosynthesis genes in Escherichia coli yields novel C11-terpenes
    Article Snippet: E . coli strain DH5α (New England Biolabs) was used for plasmid construction. .. E . coli MG1655 Δ endA Δ recA (DE3) (hereinafter referred to as E . coli MG1655) [ ] was constructed in the lab of Kristala Prather (Addgene #37854) and used for production experiments. pJBEI-6409 was constructed in the lab of Taek Soon Lee (Addgene plasmid # 47048). ..

    Article Title: Modified bacteria for enhanced bioproduction and methods of using the same
    Article Snippet: .. T-SACK was a gift from the Court lab. pJBEI-6410 was a gift from Taek Soon Lee (Addgene plasmid #47049). pORTMAGE-2 was a gift from Csaba Pál (Addgene plasmid #72677). pCP20 was obtained from lab stocks. ..

    Purification:

    Article Title: Bacterial Production of Pinene by a Laboratory-Evolved Pinene-Synthase.
    Article Snippet: Successful feeding of the substrate geranylpyrophosphate (GPP) to monoterpene synthase is critical to the efficient microbial production of monoterpenes.. Over-expression of GPP synthases, metabolic channeling from GPP synthase to terpene synthases, and down-tuning of endogenous competitors have been successfully used to increase the production of monoterpene synthesis.. Nevertheless, the production of monoterpenes has remained considerably lower than that of hemi-/ sesqui-terpenoids.



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